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Author |
Hrdlickova Kuckova, S.; Hamidi-Asl, E.; Sofer, Z.; Marvan, P.; De Wael, K.; Sanyova, J.; Janssens, K. |
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Title |
A simplified protocol for usage of new immuno-SERS probes for detection of casein, collagens and ovalbumin in cross-sections of artworks |
Type |
A1 Journal article |
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Year  |
2018 |
Publication |
Analytical methods |
Abbreviated Journal |
Anal Methods-Uk |
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Volume |
10 |
Issue |
9 |
Pages |
1054-1062 |
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Keywords |
A1 Journal article; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
Although it is now relatively straightforward to identify protein binders in works of art, their proper localization within the corresponding layer still represents a significant analytical challenge. Until now, the identification of proteins has mainly been performed by peptide mass fingerprinting using mass spectrometric methods and their localization in polished paint cross-sections have been realized by optical microscopy via the use of fluorescent stain Sypro Ruby (SR). In this work we propose a simplified protocol for immuno-surface enhanced Raman scattering (immuno-SERS) using gold nanoparticles attached to biphenyl-4,4-dithiol (BPDT) as the SERS-nanotag. These nanoparticles are easily obtainable in the lab and have been used to label multilayered mock up samples prepared as cross-sections to estimate the detection limits of the suggested method. The layers contain egg, casein, and different animal glues binders (prepared in various ratios with linseed oil or a carbohydrate component) mixed with the pigments azurite, vermilion and chalk. The sensitivity of staining agent SR is compared to that of the immuno-SERS protocol for the first time. |
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Wos |
000426696100017 |
Publication Date |
2018-02-02 |
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ISSN |
1759-9660 |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
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Impact Factor |
1.9 |
Times cited |
3 |
Open Access |
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Notes |
; The authors wish to acknowledge the Fund for Scientific Research-Flanders (FWO-Vlaanderen) for the support to act as a Postdoctoral Fellow – Pegasus of the Research Foundation – Flanders, and the grant from Specific University research (MSMT No. 20/2017) from the Czech Republic. This work was supported by the project Advanced Functional Nanorobots (reg. No. CZ.02.1.01/0.0/0.0/15_003/ 0000444 financed by the EFRR). Zdenek Sofer and Petr Marvan were supported by specific university research (MSMT No. 20-SVV/2018). ; |
Approved |
Most recent IF: 1.9 |
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Call Number |
UA @ admin @ c:irua:148803 |
Serial |
5831 |
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