%&LaTeX
\documentclass{article}

\usepackage[utf8]{inputenc}
\usepackage[T1]{fontenc}
\usepackage{textcomp}

\begin{document}

\begin{thebibliography}{1}

\bibitem{Agrawal_etal2021} {\textquotedblleft}\textit{Time to act{\textendash}assessing variations in qPCR analyses in biological nitrogen removal with examples from partial nitritation/anammox systems}{\textquotedblright}. Agrawal S, Weissbrodt DG, Annavajhala M, Jensen MM, Arroyo JMC, Wells G, Chandran K, Vlaeminck SE, Terada A, Smets BF, Lackner S, Water Research \textbf{190}, 116604 (2021).   http://doi.org/10.1016/J.WATRES.2020.116604

\end{thebibliography}

\end{document}

