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Author | Trashin, S.; De Jong, M.; Breugelmans, T.; Pilehvar, S.; De Wael, K. | ||||
Title | Label-free impedance aptasensor for major peanut allergen Ara h 1 | Type | A1 Journal article | ||
Year | 2015 | Publication | Electroanalysis | Abbreviated Journal | |
Volume | 27 | Issue | 1 | Pages | 32-37 |
Keywords | A1 Journal article; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Applied Electrochemistry & Catalysis (ELCAT) | ||||
Abstract | The peanut allergen Ara h 1 belonging to the vicilin family of seed storage proteins is known to be a suitable marker for peanut detection in foods. In the present work, we suggest an electrochemical detection of Ara h 1 using a recently selected 80-base DNA aptamer. The detection strategy relies on insulation effect of a bulky protein captured by an immobilized aptamer. The electrodes were modified by the aptamer and characterized using electrochemical impedance spectroscopy (EIS). The aptamer surface density and analytical parameters of the calibration curves for Ara h 1 were compared with the system prepared with thrombin binding aptamer (TBA) and operated in the same conditions. | ||||
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Corporate Author | Thesis | ||||
Publisher | Place of Publication | Editor | |||
Language | Wos | 000347838300004 | Publication Date | 2014-10-13 | |
Series Editor | Series Title | Abbreviated Series Title | |||
Series Volume | Series Issue | Edition | |||
ISSN | ISBN | Additional Links | UA library record; WoS full record; WoS citing articles | ||
Impact Factor | Times cited | 10 | Open Access | ||
Notes | ; ST greatly appreciates the Research Council University of Antwerp (IWS BOF UA) for funding the post-doctoral fellowship. ; | Approved | Most recent IF: NA | ||
Call Number | UA @ admin @ c:irua:119442 | Serial | 5686 | ||
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