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Author |
Blommaerts, N. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Title |
Plasmonic core shell nanoparticles : from synthesis to photocatalytic applications |
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Doctoral thesis |
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2019 |
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153 p. |
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Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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Het gebruik van plasmon-actieve nanodeeltjes heeft de laatste 10 jaar zeer veel interesse gewekt bij onderzoekers in verschillende toepassingsdomeinen zoals fotokatalyse of oppervlakte versterkte Raman spectroscopie. Er is echter een grote limiterende factor bij het gebruik van edelmetaal nanodeeltjes zoals goud en zilver en dat is de stabiliteit. Deze oxideren en aggregeren snel, zeker in oxidatieve omgeving zoals in lucht. Een interessante aanpak om plasmon-actieve nanodeeltjes te stabiliseren, is om ze te omgeven in een schil, met andere woorden om een kern-schil nanodeeltje te vormen. Er zijn een heel aantal verschillende manieren waarop kern-schil nanodeeltjes gesynthetiseerd kunnen worden. In eerste instantie werden metaal nanodeeltjes omgeven door een (dunne) TiO2 laag. Afhankelijk van de hoeveelheid TiO2 precursor kon de dikte van de laag gecontroleerd worden tot enkele nanometers dik. De stalen werden getest voor de fotokatalytische afbraak van een vaste laag stearinezuur waarbij toevoeging van 2 wt% metaal@TiO2 op P25 leidde tot een significante verbetering in afbraakefficiëntie in vergelijking met zuiver P25. Een andere manier voor het stabiliseren van metaal nanodeeltjes is door ze te omgeven met een polymeerschil. Op deze manier kon de laagdikte gecontroleerd worden met sub-nanometer controle wat een zeer belangrijke factor is voor de hoeveelheid near-field versterking dat buiten de polymeer schil kan gaan. Een XTT test werd uitgevoerd om te bepalen wat de zuurstofactivatie snelheid was van goud en zilver (en goud-zilver bimetallische) nanodeeltjes, al dan niet omgeven door een (niet-)geleidende polymeer laag. Wanneer de stalen gecoat werden met vier niet-geleidende polymeerlagen zakte de zuurstofactivatie nagenoeg tot nul. Aan de andere kant, als goud nanodeeltjes werden omgeven door een geleidende schil was er nog steeds zuurstofactivatie, hoewel lager dan in het geval van goud zonder laag. Het laatste deel van deze thesis focuste meer op mogelijke toepassingen in luchtzuivering. In dit werk werd een glazen buis, gecoat aan de binnenkant met (Ag@polymer gemodificeerd) TiO2, als een spiraal rond een UVA lamp gewikkeld. De geoptimaliseerde spiraalreactor werd dan vergeleken met een conventionele cilindervormige fotoreactor, met dezelfde dimensies en totale katalysatorbelading, over een grote range aan experimentele condities. Uit de resultaten bleek dat de spiraalreactor significant betere afbraakefficiënties vertoonde in vergelijking met de conventionele cilindervormige reactor over een grote range aan debieten. Een adsorptiestap in combinatie met de geoptimaliseerde spiraalreactor zou kunnen leiden tot een zeer krachtige luchtzuiveringstechnologie. |
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UA @ admin @ c:irua:164835 |
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8389 |
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Author |
Bencs, L.; Ravindra, K.; Van Grieken, R. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Title |
Platinum : environmental pollution and health effects |
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H2 Book chapter |
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2011 |
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580-595
T2 - Encyclopedia of environmental health |
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H2 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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978-0-444-52273-3 |
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UA @ admin @ c:irua:93109 |
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8390 |
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Trincavelli, J.; Montoro, S.; Van Grieken, R.; van Espen, P. |
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Title |
Prediction of Mα/Lα intensity ratios and the use in the spectra evaluation |
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P3 Proceeding |
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1992 |
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P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Chemometrics (Mitac 3) |
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UA @ admin @ c:irua:2863 |
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8405 |
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Author |
Tsuji, K.; Spolnik, Z.; Wagatsuma, K.; Nullens, R.; Van Grieken, R.E. |
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Title |
Preliminary experiments on grazing-exit electron probe microanalysis (GE-EPMA) |
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P1 Proceeding |
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1999 |
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119-122
T2 - Electron Microscopy and Analysis 1999 |
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P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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000088782800027 |
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UA @ admin @ c:irua:27602 |
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8408 |
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Goossenaerts, C.; Verbueken, A.; Van Grieken, R. |
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Title |
Preliminary LAMMA investigations on healthy and acid rain affected spruce needles |
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P3 Proceeding |
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1986 |
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P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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UA @ admin @ c:irua:117511 |
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8409 |
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Author |
Gielis, J.; Potters, G. |
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Title |
Proceedings of the 9th World Bamboo Congress, Antwerp 2012 |
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P3 Proceeding |
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2012 |
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P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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UA @ admin @ c:irua:97756 |
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8413 |
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Author |
Jochems, P. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Title |
Process intensification by immobilization of \beta-galactosidase on a mixed matrix membrance : galacto-oligosaccharides production as a case study |
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Doctoral thesis |
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2013 |
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199 p. |
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Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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978-90-5728-430-4 |
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UA @ admin @ c:irua:111811 |
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8415 |
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Author |
Sui, Y. |
![find book details (via ISBN) isbn](img/isbn.gif)
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Title |
Producing nutritional protein with Dunaliella microalgae : technological and economic optimization |
Type |
Doctoral thesis |
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Year |
2019 |
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140 p. |
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Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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In this thesis, microalga Dunaliella salina is highlighted as a novel source of protein to sustain the human needs. As demonstrated in this thesis, the biochemical composition of D. salina is not fixed, and can be substantially influenced by internal and external conditions. In order to comply with the human requirement of protein, various important factors affecting the protein quantity and quality of D. salina have been evaluated in this thesis for an optimized production strategy. All tested parameters, namely salinity, pH, light regimes (continuous light and light/dark cycle), light intensity, nutrient levels and growth phases can contribute to significant variations of protein content and essential amino acid (EAA) level in D. salina. Ultimately, D. salina is capable of producing high amount of superior quality protein, complying with the FAO reference for human consumption. Even better, such protein of superior quality can be accompanied by unique β-carotene accumulation in D. salina, a pigment with anti-oxidant pro-vitamin A effect. In the end, according to the techno-economic analysis (TEA), it is economically feasible to produce D. salina biomass for human nutrition. |
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978-90-5728-630-8 |
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Call Number |
UA @ admin @ c:irua:164002 |
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8420 |
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Author |
Van Grieken, R.; Wollast, R. |
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Progress in Belgian oceanographic research : proceedings of symposium, Brussels, 3-5 March 1985 |
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ME3 Book as editor |
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1985 |
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479 p. |
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ME3 Book as editor; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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UA @ admin @ c:irua:117474 |
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8422 |
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Author |
Alloul, A. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Title |
Purple bacteria as microbial protein source : technology development, community control, economic optimization and biomass valorization |
Type |
Doctoral thesis |
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2019 |
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212 p. |
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Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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Food production is a cornerstone in contemporary industrial societies. Its production requires land, water and enormous amounts of fertilizers. These precious fertilizers enter the linear food chain and suffer from a cascade of inefficiencies, resulting in detrimental effects to the environment. A radical transforming of the current food production chain is, therefore, essential to guarantee a sustainable future for humanity. This thesis has studied the production of microbial protein (i.e. single-cell protein), which is the use of microorganisms such as yeast, fungi, algae and bacteria as protein ingredient for animal feed. The type of microorganisms targeted in this thesis were purple non-sulfur bacteria (PNSB). These bacteria are an extremely heterogenic group that contain photosynthetic pigments and are able to perform anoxygenic photosynthesis. The core focus of the thesis was technology development for the production of PNSB as a source of microbial protein on wastewater and fresh fertilizers. In the final stage of this research, it was the objective to explore the potential of PNSB as a nutritious feed ingredient for shrimp. Overall, this work has provided the building blocks to transform the conventional food production chain. The findings show that PNSB production and biomass valorization is within reach. Further pilot implementation and cost reduction will facilitate the introduction of PNSB production in future’s wastewater treatment plants and the valorization of the biomass as nutritious animal feed ingredient. |
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978-90-5728-636-0 |
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UA @ admin @ c:irua:164820 |
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8430 |
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Markowicz, A.A.; Van Grieken, R.E. |
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Quantification in XRF analysis of intermediate-thickness samples |
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2002 |
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407-431
T2 - Handbook of X-ray spectrometry / Grie |
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H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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UA @ admin @ c:irua:36389 |
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8433 |
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Markowicz, A.M.; Van Grieken, R.E. |
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Quantification in XRF analysis of intermediate-thickness samples |
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1992 |
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339-358
T2 - Handbook of X-ray spectroscopy / Grie |
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H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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UA @ admin @ c:irua:2855 |
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8434 |
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Otten, P.; Bruynseels, F.; Van Grieken, R. |
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Reaction of marine aerosols with HNO3 vapour studied by single particle analysis |
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1986 |
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P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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UA @ admin @ c:irua:117508 |
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8455 |
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Claes, M.; de Bokx, P.; Van Grieken, R. |
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Recent developments of laboratory grazing emission X-ray fluorescence spectrometry |
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P1 Proceeding |
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1999 |
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103-107
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P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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000081658400015 |
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UA library record; WoS full record |
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Open Access |
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Approved |
no |
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Call Number |
UA @ admin @ c:irua:24718 |
Serial |
8459 |
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Permanent link to this record |
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Author |
Bruynseels, F.; Van Grieken, R. |
|
|
Title |
Recombination reactions and geometry effects in laser microprobe mass analysis studied with 12C/13C bilayers |
Type |
P3 Proceeding |
|
Year |
1986 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
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Abbreviated Journal |
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Volume |
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Issue |
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Pages |
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Keywords |
P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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UA library record |
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|
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|
Open Access |
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Notes |
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Approved |
no |
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|
Call Number |
UA @ admin @ c:irua:117512 |
Serial |
8462 |
|
Permanent link to this record |
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Author |
Injuk, J.; Van Grieken, R. |
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Title |
Reference matarials for microanalytical nuclear techniques |
Type |
P3 Proceeding |
|
Year |
1995 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
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Abbreviated Journal |
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Volume |
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Issue |
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Pages |
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Keywords |
P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Additional Links |
UA library record |
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|
Impact Factor |
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|
Open Access |
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|
|
Notes |
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Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:55574 |
Serial |
8467 |
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Permanent link to this record |
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Author |
Tytgat, T. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Title |
Research and development of self-supporting TiO2 foams for removal of VOCs from ambient air |
Type |
Doctoral thesis |
|
Year |
2012 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
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Abbreviated Journal |
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Volume |
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Issue |
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Pages |
164 p. |
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Keywords |
Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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|
Abbreviated Series Title |
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|
|
Series Volume |
|
Series Issue |
|
Edition |
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|
|
ISSN |
|
ISBN |
978-90-5728-395-6 |
Additional Links |
UA library record |
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|
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Times cited |
|
Open Access |
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|
|
Notes |
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Approved |
no |
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|
Call Number |
UA @ admin @ c:irua:104607 |
Serial |
8472 |
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Permanent link to this record |
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Author |
Kontozova, V.; Krupińska, B.; Van Grieken, R.; Janssen, E.; Moris, H.; Aerts, D.; Watteeuw, L.; van Bos, M.; Peckstadt, A. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Title |
Research for preventive conservation and optimal presentation of world heritage in Museum Plantin-Moretus, Antwerp |
Type |
P3 Proceeding |
|
Year |
2011 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
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Abbreviated Journal |
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Volume |
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Issue |
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Pages |
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Keywords |
P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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|
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|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
978-989-97522-0-7 |
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:92095 |
Serial |
8473 |
|
Permanent link to this record |
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Author |
Swerling, G.; Travaglini, B.; Van Grieken, R.; Spolnik, Z.; Bencs, L.; [et al.]; European Project Freindly-Heating |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Il riscaldamento nelle chiese e la conservazione dei beni culturali = Church heating and the preservation of the cultural heritage |
Type |
ME2 Book as editor or co-editor |
|
Year |
2006 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
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|
Volume |
|
Issue |
|
Pages |
240 p. |
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Keywords |
ME2 Book as editor or co-editor; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
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Address |
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Wos |
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|
|
Series Volume |
|
Series Issue |
|
Edition |
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|
ISSN |
|
ISBN |
88-370-5034-8 |
Additional Links |
UA library record |
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|
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Open Access |
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|
|
Notes |
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Approved |
no |
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|
Call Number |
UA @ admin @ c:irua:61684 |
Serial |
8480 |
|
Permanent link to this record |
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Author |
Vandekerckhove, T.G.L.; Courtens, E.N.P.; Prat, D.; Boon, N.; Vlaeminck, S.E. |
|
|
Title |
The rise of thermophilic biotechnology for nitrogen removal |
Type |
P3 Proceeding |
|
Year |
2016 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
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|
Volume |
|
Issue |
|
Pages |
17 p.
T2 - WEF/IWA Nutrient Removal and Recovery C |
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|
Keywords |
P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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|
ISBN |
|
Additional Links |
UA library record |
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|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:151125 |
Serial |
8481 |
|
Permanent link to this record |
|
|
|
|
Author |
Moropoulou, A.; Bisbikou, K.; Torfs, K.; Van Grieken, R. |
|
|
Title |
Risk analysis for the protection of cultural heritage from industrial pollution |
Type |
H3 Book chapter |
|
Year |
1999 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
475-484
T2 - Structural studies, repairs and maint |
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|
Keywords |
H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
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|
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Edition |
|
|
|
ISSN |
|
ISBN |
|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:27598 |
Serial |
8483 |
|
Permanent link to this record |
|
|
|
|
Author |
Chou, L.; Harlay, J.; Roevros, N.; Lannuzel, D.; Rebreanu, L.; van der Zee, C.; Lapernat, P.-E.; Daro, M.-H.; Aerts, K.; Godoi, R.; Van Grieken, R. |
|
|
Title |
Role of oceanic production and dissolution of calcium carbonate in climate change (CCCC): final report |
Type |
Minutes and reports |
|
Year |
2009 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
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Abbreviated Journal |
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Volume |
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Issue |
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Pages |
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Keywords |
Minutes and reports; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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|
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|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:80696 |
Serial |
8486 |
|
Permanent link to this record |
|
|
|
|
Author |
Delalieux, F.; Van Grieken, R.; Moropoulou, A. |
|
|
Title |
Salt weathering research: present state and future directions |
Type |
P3 Proceeding |
|
Year |
2003 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
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Volume |
|
Issue |
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Pages |
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|
Keywords |
P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
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Edition |
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|
|
ISSN |
|
ISBN |
|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:42732 |
Serial |
8490 |
|
Permanent link to this record |
|
|
|
|
Author |
van der Snickt, G.; de Boeck, A.; Keutgens, K.; Anthierens, D. |
|
|
Title |
The SALUT project : study of advanced laser techniques for the uncovering of polychromed works of art |
Type |
P1 Proceeding |
|
Year |
2007 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
|
|
|
Volume |
116 |
Issue |
|
Pages |
151-158
T2 - Proceedings of the 6th International |
|
|
Keywords |
P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
|
|
Abstract |
In order to find out whether the existing laser systems can be employed to remove superimposed layers of paint on secco wall paintings in a selective way, laser tests were carried out on three types of prepared samples simulating three stratigraphies that are frequently encountered in practice. OM, EPMA, colorimetry, mu Raman, and FT-IR were used to evaluate the results. It was found that Q-switched Nd:YAG lasers emitting at 1,064 nm could be employed to remove unwanted layers of oil paint and limewash, but the treatment of large areas requires implementation of a computer-controlled X-Y-Z station in order to control the parameters. However, the applicability of this technique will remain limited as ablation at the established optimum parameters implied a discoloration of the pigments cinnabar, yellow ochre, and burnt sienna. Moreover, it was observed that no ablation took place when the limewash thickness exceeds 25 mu m. Unwanted layers of acrylic could be removed in an efficient way with an excimer laser emitting at 193 nm. |
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000252680500018 |
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|
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|
Abbreviated Series Title |
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|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
978-3-540-72129-1; 0930-8989 |
ISBN |
|
Additional Links |
UA library record; WoS full record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:157216 |
Serial |
8491 |
|
Permanent link to this record |
|
|
|
|
Author |
Schmeling, M.; Van Grieken, R.E. |
|
|
Title |
Sample preparation for X-ray fluorescence |
Type |
H3 Book chapter |
|
Year |
2002 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
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|
|
Volume |
|
Issue |
|
Pages |
933-976
T2 - Handbook of X-ray spectrometry / Grie |
|
|
Keywords |
H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
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Edition |
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|
|
ISSN |
|
ISBN |
|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:36388 |
Serial |
8494 |
|
Permanent link to this record |
|
|
|
|
Author |
Injuk, J.; Van Grieken, R. |
|
|
Title |
Sample preparation for X-ray fluorescence analysis |
Type |
H3 Book chapter |
|
Year |
2000 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
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|
|
Volume |
15 |
Issue |
|
Pages |
13338-13347
T2 - Encyclopedia of analytical chemis |
|
|
Keywords |
H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
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Address |
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|
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Series Issue |
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Edition |
|
|
|
ISSN |
|
ISBN |
|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:31656 |
Serial |
8495 |
|
Permanent link to this record |
|
|
|
|
Author |
Injuk, J.; Van Grieken, R.E. |
|
|
Title |
Sample preparation for XRF |
Type |
H3 Book chapter |
|
Year |
1992 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
657-692
T2 - Handbook of X-ray spectroscopy / Grie |
|
|
Keywords |
H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
|
|
Abstract |
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Address |
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Place of Publication |
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Wos |
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Series Editor |
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|
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Series Volume |
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Series Issue |
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Edition |
|
|
|
ISSN |
|
ISBN |
|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:2857 |
Serial |
8496 |
|
Permanent link to this record |
|
|
|
|
Author |
Robberecht, H.; Van Grieken, R. |
|
|
Title |
Selenium content and speciation in environmental waters determied by X-ray fluorescence spectroscopy |
Type |
H3 Book chapter |
|
Year |
1980 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
362-366
T2 - Trace substances in environmental hea |
|
|
Keywords |
H3 Book chapter; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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|
Abstract |
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Address |
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Edition |
|
|
|
ISSN |
|
ISBN |
|
Additional Links |
UA library record |
|
|
Impact Factor |
|
Times cited |
|
Open Access |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number |
UA @ admin @ c:irua:117481 |
Serial |
8505 |
|
Permanent link to this record |
|
|
|
|
Author |
vanden Berghe, D.; Deelstra, H.; Robberecht, H.; Van Grieken, R. |
|
|
Title |
Selenium content of soils and rye grass (Lolium multiflorum) in Belgium |
Type |
H3 Book chapter |
|
Year |
1981 |
Publication ![sorted by Publication field, descending order (down)](img/sort_desc.gif) |
|
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
85-105
T2 - Mineral elements '80 : part 1 |
|
|
Keywords |
H3 Book chapter; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
|
|
Abstract |
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Address |
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Wos |
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UA @ admin @ c:irua:117483 |
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8506 |
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Daems, E. |
![find record details (via OpenURL) openurl](img/xref.gif)
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Shaping up oligonucleotides : aptamer-target recognition investigated by native mass spectrometry |
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Doctoral thesis |
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2021 |
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235 p. |
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Doctoral thesis; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Aptamers are short, synthetic DNA or RNA molecules that are characterized by a specific 3D conformation which enables specific target recognition. Aptamers are promising tools in many application fields from sensing to therapeutics. One of the major challenges in the aptamer field is understanding the relationship between the sequence and what determines the higher-order structure and specific interactions with targets. Therefore, this PhD thesis focuses on the use of different mass spectrometry (MS) based approaches to characterize aptamers and their interactions. Several of these approaches are already widely applied to study other biomolecules, such as proteins, but are still largely unexplored for aptamers and oligonucleotides in general. A first focus was put on obtaining information on the higher-order structure and conformational stability of aptamers using a combination of MS and with ion mobility (IM) spectrometry by performing collision-induced unfolding (CIU) experiments. CIU was shown to hold great promise to analyze the conformational dynamics and gas-phase stabilities of aptamers. Next, the capabilities and limitations of native IM-MS for the analysis of noncovalent interactions of aptamers were demonstrated. The conformational behavior and interactions of cocaine-binding aptamers were studied and it was found that relative binding affinities of aptamers that only differ slightly in sequence and structure can be determined using native MS. Moreover, native IM-MS allowed the detection of small conformational changes upon binding of a target, which were found to be dependent on the binding mode of the aptamer. An adaptive binding mechanism was suggested for flexible aptamers that require more reorganization upon binding. In the final part of this thesis, the importance of thoroughly characterizing and validating aptamer-target interactions before using them in an application was emphasized. Moreover, the gathered insights were applied in our own development of a proof-of-concept aptamer-based sensor. This was shown by investigating the interactions of ampicillin aptamers which were found to not bind the target they were selected for in the first place. A multi-analytical approach combining complementary techniques was used for this purpose since no single technique is generally applicable to characterize all aptamers and their interactions and to obtain a comprehensive picture of the aptamer-target interactions. Furthermore, such multi-analytical approach was used to characterize a testosterone-binding aptamer while developing an aptamer-based electrochemiluminescent sensing strategy for this target. This shows the importance of native MS, in combination with other techniques, to thoroughly understand the aptamer-target interactions in the development of a designed application. |
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Most recent IF: NA |
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UA @ admin @ c:irua:178116 |
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8517 |
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