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“Sample preparation for X-ray fluorescence analysis”. Injuk J, Van Grieken R page 13338 (2000).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Sample preparation for XRF”. Injuk J, Van Grieken RE page 657 (1992).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Selenium content and speciation in environmental waters determied by X-ray fluorescence spectroscopy”. Robberecht H, Van Grieken R page 362 (1980).
Keywords: H3 Book chapter; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Selenium content of soils and rye grass (Lolium multiflorum) in Belgium”. vanden Berghe D, Deelstra H, Robberecht H, Van Grieken R page 85 (1981).
Keywords: H3 Book chapter; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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Daems E (2021) Shaping up oligonucleotides : aptamer-target recognition investigated by native mass spectrometry. 235 p
Abstract: Aptamers are short, synthetic DNA or RNA molecules that are characterized by a specific 3D conformation which enables specific target recognition. Aptamers are promising tools in many application fields from sensing to therapeutics. One of the major challenges in the aptamer field is understanding the relationship between the sequence and what determines the higher-order structure and specific interactions with targets. Therefore, this PhD thesis focuses on the use of different mass spectrometry (MS) based approaches to characterize aptamers and their interactions. Several of these approaches are already widely applied to study other biomolecules, such as proteins, but are still largely unexplored for aptamers and oligonucleotides in general. A first focus was put on obtaining information on the higher-order structure and conformational stability of aptamers using a combination of MS and with ion mobility (IM) spectrometry by performing collision-induced unfolding (CIU) experiments. CIU was shown to hold great promise to analyze the conformational dynamics and gas-phase stabilities of aptamers. Next, the capabilities and limitations of native IM-MS for the analysis of noncovalent interactions of aptamers were demonstrated. The conformational behavior and interactions of cocaine-binding aptamers were studied and it was found that relative binding affinities of aptamers that only differ slightly in sequence and structure can be determined using native MS. Moreover, native IM-MS allowed the detection of small conformational changes upon binding of a target, which were found to be dependent on the binding mode of the aptamer. An adaptive binding mechanism was suggested for flexible aptamers that require more reorganization upon binding. In the final part of this thesis, the importance of thoroughly characterizing and validating aptamer-target interactions before using them in an application was emphasized. Moreover, the gathered insights were applied in our own development of a proof-of-concept aptamer-based sensor. This was shown by investigating the interactions of ampicillin aptamers which were found to not bind the target they were selected for in the first place. A multi-analytical approach combining complementary techniques was used for this purpose since no single technique is generally applicable to characterize all aptamers and their interactions and to obtain a comprehensive picture of the aptamer-target interactions. Furthermore, such multi-analytical approach was used to characterize a testosterone-binding aptamer while developing an aptamer-based electrochemiluminescent sensing strategy for this target. This shows the importance of native MS, in combination with other techniques, to thoroughly understand the aptamer-target interactions in the development of a designed application.
Keywords: Doctoral thesis; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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Piñ,era Herná,ndez I (2014) Simulation of atom displacements induced by photons and electrons in solids. 137 p
Keywords: Doctoral thesis; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Single particle analysis techniques”. de Bock LA, Van Grieken RE page 243 (1999).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Single particle characterisation of inorganic North Sea suspensions”. Jambers W, Van Grieken R, (1996)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Some new applications of auxiliary signals in X-ray fluorescence and electron microprobe analysis”. Kuczumow A, Vekemans B, Schalm O, Vincze L, Dorriné, W, Gysels K, Van Grieken R, , 197 (1999)
Keywords: P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Some studies of the effect of indoor and outdoor pollutants on cultural heritage items”. Van Grieken R, Kontozova V, Godoi RHM, Spolnik Z, Worobiec A, Deutsch F, Bencs L, (2005)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Laboratory Experimental Medicine and Pediatrics (LEMP)
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“Source apportionment of individual aerosol particles at Hungarian background stations”. Török S, Szandor S, Xhoffer C, Van Grieken R page 32 (1993).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Spark source mass spectrometry for trace analysis of diverse biological matrices”. Vos L, Van Grieken R page 303 (1983).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Spatial and temporal variation of anthropogenic palladium in the environment”. Bencs L, Ravindra K, Van Grieken R page 433 (2006).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Spatial and temporal variations in particulate Polycyclic Aromatic Hydrocarbon (PAH) levels over Menen (Belgium) and their relation with air mass trajectories”. Ravindra K, Wauters E, Van Grieken R page 838 (2007).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Speciation and distribution of sulfur and nitrogen in individual aerosol particles measured by LAMMA”. Bruynseels F, Van Grieken R, (1984)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Speciation and surface analysis of single particles using electron-excited X-ray emission spectrometry”. Szalóki I, Ro C-U, Osán J, de Hoog J, Van Grieken R page 569 (2004).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Specimen preparation”. Injuk J, Van Grieken R, Blank A, Eksperiandova L, Buhrke V page 411 (2006).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“State-of-the-art and research needs for trace metals”. Playford K, Colin JL, Pacyna JM, Pirrone N, Schulz M, Van Grieken R, Wrembel Z page 7 (1998).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Stof : begrippen, bronnen en analysmethoden”. Van Grieken R, (1985)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Structural heterogeneity within airborne particles”. Injuk J, de Bock L, Van Grieken R page 173 (1998).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Studia sezonowych zmian wewnetrznego składu powietrza atmosferycznego w kościołach zabytkowych w kontekście doboru i modyfikacji metod konserwacji obiektów zabytkowych”. Samek L, Worobiec A, Spolnik Z, Van Grieken R page 59 (2005).
Keywords: H3 Book chapter; Laboratory Experimental Medicine and Pediatrics (LEMP); AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of environmental effects on deterioration of monuments: case study the cathedral of Bari, Italy”. Zezza F, Torfs K, Van Grieken R, García Pascua N, Macri F, (1996)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of individual estuarine suspension particles by automated electron-probe X-ray microanalysis”. Bernard P, Van Grieken R, Eisma D, (1985)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of individual particle types and heavy metal deposition for North Sea aerosols using micro and trace analysis techniques”. Van Grieken R, Injuk J, de Bock L, van Malderen H page 105 (2000).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of individual particle types and heavy metal deposition for North Sea aerosols using micro- and trace analysis techniques”. Injuk J, de Bock L, van Malderen H, Van Grieken R, (1996)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of microgeometry of porous materials using synchrotron computed microtomography”. Jones KW, Feng H, Lindquist WB, Adler PM, Thover JF, Vekemans B, Vincze L, Szalóki I, Van Grieken R, Adams F, Riekel C page 39 (2003).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of the heavy metal concentration, deposition and sources of the North Sea aerosols using X-ray emission techniques”. Injuk J, van Malderen H, Van Grieken R page 793 (1993).
Keywords: H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Study of the main physical processes contributing to image formation in emission radiography using mathematical modeling”. Leyva Pernia D, Cabal Rodríguez AE, Schalm O, van Espen P, Piñera Hernández I, Abreu Alfonso Y, (2013)
Keywords: P3 Proceeding; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Antwerp Cultural Heritage Sciences (ARCHES)
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“Studying the composition of atmospheric aerosols through the joint application of hierarchical, non-hierarchical and fuzzy clustering to EPXMA data sets”. Treiger B, Bondarenko I, van Malderen H, Van Grieken R, (1996)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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“Sub-minute determination of BTEX in environmental samples using low pressure GC-ITMS”. Joos PE, Godoi AFL, de Jong R, de Zeeuw J, Van Grieken R, (2002)
Keywords: P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
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