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Author Trincavelli, J.; Montoro, S.; Van Grieken, R.; van Espen, P.
Title Prediction of Mα/Lα intensity ratios and the use in the spectra evaluation Type P3 Proceeding
Year 1992 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Chemometrics (Mitac 3)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:2863 Serial 8405
Permanent link to this record
 
 
Author Tsuji, K.; Spolnik, Z.; Wagatsuma, K.; Nullens, R.; Van Grieken, R.E.
Title Preliminary experiments on grazing-exit electron probe microanalysis (GE-EPMA) Type P1 Proceeding
Year 1999 Publication Abbreviated Journal
Volume Issue Pages 119-122 T2 - Electron Microscopy and Analysis 1999
Keywords P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos 000088782800027 Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record; WoS full record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:27602 Serial 8408
Permanent link to this record
 
 
Author Goossenaerts, C.; Verbueken, A.; Van Grieken, R.
Title Preliminary LAMMA investigations on healthy and acid rain affected spruce needles Type P3 Proceeding
Year 1986 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:117511 Serial 8409
Permanent link to this record
 
 
Author Gielis, J.; Potters, G.
Title Proceedings of the 9th World Bamboo Congress, Antwerp 2012 Type P3 Proceeding
Year 2012 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:97756 Serial 8413
Permanent link to this record
 
 
Author Jochems, P.
Title Process intensification by immobilization of \beta-galactosidase on a mixed matrix membrance : galacto-oligosaccharides production as a case study Type Doctoral thesis
Year 2013 Publication Abbreviated Journal
Volume Issue Pages 199 p.
Keywords Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-90-5728-430-4 Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:111811 Serial 8415
Permanent link to this record
 
 
Author Sui, Y.
Title Producing nutritional protein with Dunaliella microalgae : technological and economic optimization Type Doctoral thesis
Year 2019 Publication Abbreviated Journal
Volume Issue Pages 140 p.
Keywords Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract ​In this thesis, microalga Dunaliella salina is highlighted as a novel source of protein to sustain the human needs. As demonstrated in this thesis, the biochemical composition of D. salina is not fixed, and can be substantially influenced by internal and external conditions. In order to comply with the human requirement of protein, various important factors affecting the protein quantity and quality of D. salina have been evaluated in this thesis for an optimized production strategy. All tested parameters, namely salinity, pH, light regimes (continuous light and light/dark cycle), light intensity, nutrient levels and growth phases can contribute to significant variations of protein content and essential amino acid (EAA) level in D. salina. Ultimately, D. salina is capable of producing high amount of superior quality protein, complying with the FAO reference for human consumption. Even better, such protein of superior quality can be accompanied by unique β-carotene accumulation in D. salina, a pigment with anti-oxidant pro-vitamin A effect. In the end, according to the techno-economic analysis (TEA), it is economically feasible to produce D. salina biomass for human nutrition.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-90-5728-630-8 Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:164002 Serial 8420
Permanent link to this record
 
 
Author Van Grieken, R.; Wollast, R.
Title Progress in Belgian oceanographic research : proceedings of symposium, Brussels, 3-5 March 1985 Type ME3 Book as editor
Year 1985 Publication Abbreviated Journal
Volume Issue Pages 479 p.
Keywords ME3 Book as editor; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:117474 Serial 8422
Permanent link to this record
 
 
Author Alloul, A.
Title Purple bacteria as microbial protein source : technology development, community control, economic optimization and biomass valorization Type Doctoral thesis
Year 2019 Publication Abbreviated Journal
Volume Issue Pages 212 p.
Keywords Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract ​Food production is a cornerstone in contemporary industrial societies. Its production requires land, water and enormous amounts of fertilizers. These precious fertilizers enter the linear food chain and suffer from a cascade of inefficiencies, resulting in detrimental effects to the environment. A radical transforming of the current food production chain is, therefore, essential to guarantee a sustainable future for humanity. ​This thesis has studied the production of microbial protein (i.e. single-cell protein), which is the use of microorganisms such as yeast, fungi, algae and bacteria as protein ingredient for animal feed. The type of microorganisms targeted in this thesis were purple non-sulfur bacteria (PNSB). These bacteria are an extremely heterogenic group that contain photosynthetic pigments and are able to perform anoxygenic photosynthesis. The core focus of the thesis was technology development for the production of PNSB as a source of microbial protein on wastewater and fresh fertilizers. In the final stage of this research, it was the objective to explore the potential of PNSB as a nutritious feed ingredient for shrimp. ​Overall, this work has provided the building blocks to transform the conventional food production chain. The findings show that PNSB production and biomass valorization is within reach. Further pilot implementation and cost reduction will facilitate the introduction of PNSB production in future’s wastewater treatment plants and the valorization of the biomass as nutritious animal feed ingredient.​
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-90-5728-636-0 Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:164820 Serial 8430
Permanent link to this record
 
 
Author Markowicz, A.A.; Van Grieken, R.E.
Title Quantification in XRF analysis of intermediate-thickness samples Type H3 Book chapter
Year 2002 Publication Abbreviated Journal
Volume Issue Pages 407-431 T2 - Handbook of X-ray spectrometry / Grie
Keywords H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:36389 Serial 8433
Permanent link to this record
 
 
Author Markowicz, A.M.; Van Grieken, R.E.
Title Quantification in XRF analysis of intermediate-thickness samples Type H3 Book chapter
Year 1992 Publication Abbreviated Journal
Volume Issue Pages 339-358 T2 - Handbook of X-ray spectroscopy / Grie
Keywords H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:2855 Serial 8434
Permanent link to this record
 
 
Author Otten, P.; Bruynseels, F.; Van Grieken, R.
Title Reaction of marine aerosols with HNO3 vapour studied by single particle analysis Type P3 Proceeding
Year 1986 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:117508 Serial 8455
Permanent link to this record
 
 
Author Claes, M.; de Bokx, P.; Van Grieken, R.
Title Recent developments of laboratory grazing emission X-ray fluorescence spectrometry Type P1 Proceeding
Year 1999 Publication Abbreviated Journal
Volume Issue Pages 103-107 T2 - Proceedings of the European Conferenc
Keywords P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos 000081658400015 Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record; WoS full record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:24718 Serial 8459
Permanent link to this record
 
 
Author Bruynseels, F.; Van Grieken, R.
Title Recombination reactions and geometry effects in laser microprobe mass analysis studied with 12C/13C bilayers Type P3 Proceeding
Year 1986 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:117512 Serial 8462
Permanent link to this record
 
 
Author Injuk, J.; Van Grieken, R.
Title Reference matarials for microanalytical nuclear techniques Type P3 Proceeding
Year 1995 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:55574 Serial 8467
Permanent link to this record
 
 
Author Tytgat, T.
Title Research and development of self-supporting TiO2 foams for removal of VOCs from ambient air Type Doctoral thesis
Year 2012 Publication Abbreviated Journal
Volume Issue Pages 164 p.
Keywords Doctoral thesis; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-90-5728-395-6 Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:104607 Serial 8472
Permanent link to this record
 
 
Author Kontozova, V.; Krupińska, B.; Van Grieken, R.; Janssen, E.; Moris, H.; Aerts, D.; Watteeuw, L.; van Bos, M.; Peckstadt, A.
Title Research for preventive conservation and optimal presentation of world heritage in Museum Plantin-Moretus, Antwerp Type P3 Proceeding
Year 2011 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-989-97522-0-7 Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:92095 Serial 8473
Permanent link to this record
 
 
Author Swerling, G.; Travaglini, B.; Van Grieken, R.; Spolnik, Z.; Bencs, L.; [et al.]; European Project Freindly-Heating
Title Il riscaldamento nelle chiese e la conservazione dei beni culturali = Church heating and the preservation of the cultural heritage Type ME2 Book as editor or co-editor
Year 2006 Publication Abbreviated Journal
Volume Issue Pages 240 p.
Keywords ME2 Book as editor or co-editor; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 88-370-5034-8 Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:61684 Serial 8480
Permanent link to this record
 
 
Author Vandekerckhove, T.G.L.; Courtens, E.N.P.; Prat, D.; Boon, N.; Vlaeminck, S.E.
Title The rise of thermophilic biotechnology for nitrogen removal Type P3 Proceeding
Year 2016 Publication Abbreviated Journal
Volume Issue Pages 17 p. T2 - WEF/IWA Nutrient Removal and Recovery C
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151125 Serial 8481
Permanent link to this record
 
 
Author Moropoulou, A.; Bisbikou, K.; Torfs, K.; Van Grieken, R.
Title Risk analysis for the protection of cultural heritage from industrial pollution Type H3 Book chapter
Year 1999 Publication Abbreviated Journal
Volume Issue Pages 475-484 T2 - Structural studies, repairs and maint
Keywords H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:27598 Serial 8483
Permanent link to this record
 
 
Author Chou, L.; Harlay, J.; Roevros, N.; Lannuzel, D.; Rebreanu, L.; van der Zee, C.; Lapernat, P.-E.; Daro, M.-H.; Aerts, K.; Godoi, R.; Van Grieken, R.
Title Role of oceanic production and dissolution of calcium carbonate in climate change (CCCC): final report Type Minutes and reports
Year 2009 Publication Abbreviated Journal
Volume Issue Pages
Keywords Minutes and reports; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:80696 Serial 8486
Permanent link to this record
 
 
Author Delalieux, F.; Van Grieken, R.; Moropoulou, A.
Title Salt weathering research: present state and future directions Type P3 Proceeding
Year 2003 Publication Abbreviated Journal
Volume Issue Pages
Keywords P3 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:42732 Serial 8490
Permanent link to this record
 
 
Author van der Snickt, G.; de Boeck, A.; Keutgens, K.; Anthierens, D.
Title The SALUT project : study of advanced laser techniques for the uncovering of polychromed works of art Type P1 Proceeding
Year 2007 Publication Abbreviated Journal
Volume 116 Issue Pages 151-158 T2 - Proceedings of the 6th International
Keywords P1 Proceeding; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract In order to find out whether the existing laser systems can be employed to remove superimposed layers of paint on secco wall paintings in a selective way, laser tests were carried out on three types of prepared samples simulating three stratigraphies that are frequently encountered in practice. OM, EPMA, colorimetry, mu Raman, and FT-IR were used to evaluate the results. It was found that Q-switched Nd:YAG lasers emitting at 1,064 nm could be employed to remove unwanted layers of oil paint and limewash, but the treatment of large areas requires implementation of a computer-controlled X-Y-Z station in order to control the parameters. However, the applicability of this technique will remain limited as ablation at the established optimum parameters implied a discoloration of the pigments cinnabar, yellow ochre, and burnt sienna. Moreover, it was observed that no ablation took place when the limewash thickness exceeds 25 mu m. Unwanted layers of acrylic could be removed in an efficient way with an excimer laser emitting at 193 nm.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos 000252680500018 Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 978-3-540-72129-1; 0930-8989 ISBN Additional Links UA library record; WoS full record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:157216 Serial 8491
Permanent link to this record
 
 
Author Schmeling, M.; Van Grieken, R.E.
Title Sample preparation for X-ray fluorescence Type H3 Book chapter
Year 2002 Publication Abbreviated Journal
Volume Issue Pages 933-976 T2 - Handbook of X-ray spectrometry / Grie
Keywords H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:36388 Serial 8494
Permanent link to this record
 
 
Author Injuk, J.; Van Grieken, R.
Title Sample preparation for X-ray fluorescence analysis Type H3 Book chapter
Year 2000 Publication Abbreviated Journal
Volume 15 Issue Pages 13338-13347 T2 - Encyclopedia of analytical chemis
Keywords H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:31656 Serial 8495
Permanent link to this record
 
 
Author Injuk, J.; Van Grieken, R.E.
Title Sample preparation for XRF Type H3 Book chapter
Year 1992 Publication Abbreviated Journal
Volume Issue Pages 657-692 T2 - Handbook of X-ray spectroscopy / Grie
Keywords H3 Book chapter; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:2857 Serial 8496
Permanent link to this record
 
 
Author Robberecht, H.; Van Grieken, R.
Title Selenium content and speciation in environmental waters determied by X-ray fluorescence spectroscopy Type H3 Book chapter
Year 1980 Publication Abbreviated Journal
Volume Issue Pages 362-366 T2 - Trace substances in environmental hea
Keywords H3 Book chapter; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:117481 Serial 8505
Permanent link to this record
 
 
Author vanden Berghe, D.; Deelstra, H.; Robberecht, H.; Van Grieken, R.
Title Selenium content of soils and rye grass (Lolium multiflorum) in Belgium Type H3 Book chapter
Year 1981 Publication Abbreviated Journal
Volume Issue Pages 85-105 T2 - Mineral elements '80 : part 1
Keywords H3 Book chapter; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:117483 Serial 8506
Permanent link to this record
 
 
Author Daems, E.
Title Shaping up oligonucleotides : aptamer-target recognition investigated by native mass spectrometry Type Doctoral thesis
Year 2021 Publication Abbreviated Journal
Volume Issue Pages 235 p.
Keywords Doctoral thesis; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Abstract Aptamers are short, synthetic DNA or RNA molecules that are characterized by a specific 3D conformation which enables specific target recognition. Aptamers are promising tools in many application fields from sensing to therapeutics. One of the major challenges in the aptamer field is understanding the relationship between the sequence and what determines the higher-order structure and specific interactions with targets. Therefore, this PhD thesis focuses on the use of different mass spectrometry (MS) based approaches to characterize aptamers and their interactions. Several of these approaches are already widely applied to study other biomolecules, such as proteins, but are still largely unexplored for aptamers and oligonucleotides in general. A first focus was put on obtaining information on the higher-order structure and conformational stability of aptamers using a combination of MS and with ion mobility (IM) spectrometry by performing collision-induced unfolding (CIU) experiments. CIU was shown to hold great promise to analyze the conformational dynamics and gas-phase stabilities of aptamers. Next, the capabilities and limitations of native IM-MS for the analysis of noncovalent interactions of aptamers were demonstrated. The conformational behavior and interactions of cocaine-binding aptamers were studied and it was found that relative binding affinities of aptamers that only differ slightly in sequence and structure can be determined using native MS. Moreover, native IM-MS allowed the detection of small conformational changes upon binding of a target, which were found to be dependent on the binding mode of the aptamer. An adaptive binding mechanism was suggested for flexible aptamers that require more reorganization upon binding. In the final part of this thesis, the importance of thoroughly characterizing and validating aptamer-target interactions before using them in an application was emphasized. Moreover, the gathered insights were applied in our own development of a proof-of-concept aptamer-based sensor. This was shown by investigating the interactions of ampicillin aptamers which were found to not bind the target they were selected for in the first place. A multi-analytical approach combining complementary techniques was used for this purpose since no single technique is generally applicable to characterize all aptamers and their interactions and to obtain a comprehensive picture of the aptamer-target interactions. Furthermore, such multi-analytical approach was used to characterize a testosterone-binding aptamer while developing an aptamer-based electrochemiluminescent sensing strategy for this target. This shows the importance of native MS, in combination with other techniques, to thoroughly understand the aptamer-target interactions in the development of a designed application.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved Most recent IF: NA
Call Number UA @ admin @ c:irua:178116 Serial 8517
Permanent link to this record
 
 
Author De Cocker, P.; Bessiere, Y.; Hernandez-Raquet, G.; Dubos, S.; Mercade, M.; Sun, X.Y.; Mozo, I.; Barillon, B.; Gaval, G.; Caligaris, M.; Martin Ruel, S.; Vlaeminck, S.E.; Sperandio, M.
Title Short and long term effect of decreasing temperature on anammox activity and enrichment in mainstream granular sludge process Type P1 Proceeding
Year 2017 Publication Abbreviated Journal
Volume 4 Issue Pages 50-54 T2 - Frontiers International Conference on W
Keywords P1 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract This study investigates the impact of lower temperature on short term and long term (down to 10 degrees C) on a completely anoxic anammox granular sludge process. This is the first time granular sludge Anammox is operated in pure anoxic condition in SBR and at low temperature. Conversion performance, kinetic parameters, sludge characteristics and microbial community were analyzed.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date 2017-05-04
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151120 Serial 8519
Permanent link to this record
 
 
Author Zhang, Q.; De Clippeleir, H.; Al-Omari, A.; Wett, B.; Vlaeminck, S.E.; Murthy, S.
Title Sidestream deammonification on thermal hydrolysis process digestate : strategies to overcome nitritation inhibition Type P3 Proceeding
Year 2015 Publication Abbreviated Journal
Volume Issue Pages 11 p. T2 - IWA Nutrient Removal and Recovery 2015:
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language (up) Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151147 Serial 8522
Permanent link to this record