| Records |
| Author |
Christiaens, M.E.R.; De Paepe, J.; Ilgrande, C.; De Vrieze, J.; Barys, J.; Teirlinck, P.; Meerbergen, K.; Lievens, B.; Boon, N.; Clauwaert, P.; Vlaeminck, S.E. |
| Title |
Urine nitrification with a synthetic microbial community |
Type |
A1 Journal article |
| Year |
2019 |
Publication |
Systematic and applied microbiology |
Abbreviated Journal |
|
| Volume |
42 |
Issue |
6 |
Pages |
Unsp 126021 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
During long-term extra-terrestrial missions, food is limited and waste is generated. By recycling valuable nutrients from this waste via regenerative life support systems, food can be produced in space. Astronauts' urine can, for instance, be nitrified by micro-organisms into a liquid nitrate fertilizer for plant growth in space. Due to stringent conditions in space, microbial communities need to be be defined (gnotobiotic); therefore, synthetic rather than mixed microbial communities are preferred. For urine nitrification, synthetic communities face challenges, such as from salinity, ureolysis, and organics. In this study, a synthetic microbial community containing an AOB (Nitrosomonas europaea), NOB (Nitrobacter winogradskyi), and three ureolytic heterotrophs (Pseudomonas fluorescens, Acidovorax delafieldii, and Delftia acidovorans) was compiled and evaluated for these challenges. In reactor 1, salt adaptation of the ammonium-fed AOB and NOB co-culture was possible up to 45 mS cm(-1), which resembled undiluted nitrified urine, while maintaining a 44 +/- 10 mg NH4+-N L-1 d(-1) removal rate. In reactor 2, the nitrifiers and ureolytic heterotrophs were fed with urine and achieved a 15 +/- 6 mg NO3--N L-1 d(-1) production rate for 1% and 10% synthetic and fresh real urine, respectively. Batch activity tests with this community using fresh real urine even reached 29 +/- 3 mg N L-1 d(-1). Organics removal in the reactor (69 +/- 15%) should be optimized to generate a nitrate fertilizer for future space applications. (C) 2019 Elsevier GmbH. All rights reserved. |
| Address |
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Place of Publication |
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| Language |
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Wos |
000494650600006 |
Publication Date |
2019-09-23 |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
0723-2020; 1618-0984 |
ISBN |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
Impact Factor  |
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Times cited |
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Open Access |
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| Notes |
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Approved |
no |
| Call Number |
UA @ admin @ c:irua:164650 |
Serial |
8717 |
| Permanent link to this record |
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| |
| Author |
Verstraete, W.; Clauwaert, P.; Vlaeminck, S.E. |
| Title |
Used water and nutrients : recovery perspectives in a 'panta rhei' context |
Type |
A1 Journal article |
| Year |
2016 |
Publication |
Bioresource technology |
Abbreviated Journal |
|
| Volume |
215 |
Issue |
|
Pages |
199-208 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
There is an urgent need to secure global supplies in safe water and proteinaceous food in an eco-sustainable manner, as manifested from tensions in the nexus Nutrients-Energy-Water-Environment-Land. This paper is concept based and provides solutions based on resource recovery from municipal and industrial wastewater and from manure. A set of decisive factors is reviewed facilitating an attractive business case. Our key message is that a robust barrier must clear the recovered product from its original status. Besides refined inorganic fertilizers, a central role for five types of microbial protein is proposed. A resource cycling solution for the extremely confined environment of space habitation should serve as an incentive to assimilate a new user mindset. To achieve the ambitious goal of sustainable food security, the solutions suggested here need a broad implementation, hand in hand with minimizing losses along the entire fertilizer-feed-food-fork chain. (C) 2016 Elsevier Ltd. All rights reserved. |
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Wos |
000377935100022 |
Publication Date |
2016-04-29 |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
0960-8524 |
ISBN |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
| Impact Factor |
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Times cited |
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Open Access |
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| Notes |
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Approved |
no |
| Call Number |
UA @ admin @ c:irua:134591 |
Serial |
8726 |
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| |
| Author |
Alloul, A.; Wuyts, S.; Lebeer, S.; Vlaeminck, S.E. |
| Title |
Volatile fatty acids impacting phototrophic growth kinetics of purple bacteria : paving the way for protein production on fermented wastewater |
Type |
A1 Journal article |
| Year |
2019 |
Publication |
Water research |
Abbreviated Journal |
|
| Volume |
152 |
Issue |
|
Pages |
138-147 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
Nutrient losses in our food chain severely surpass our planetary boundaries. Resource recovery can contribute to mitigation, for instance through converting wastewater resources to microbial protein for animal feed. Wastewater typically holds a complex mixture of organics, posing a challenge to selectively produce heterotrophic biomass. Ensuring the product's quality could be achieved by anaerobic generation of volatile fatty acids (VFAs) followed by photoheterotrophic production of purple non-sulfur bacteria (PNSB) with infrared light. This study aimed to determine the most suitable PNSB culture for VFA conversion and map the effect of acetate, propionate, butyrate and a VFA mixture on growth and biomass yield. Six cultures were screened in batch: (i) Rhodopseudomonas palustris, (ii) Rhodobacter sphaeroides, (iii) Rhodospirillum rubrum, (iv) a 3-species synthetic community (i+ii+iii), (v) a community enriched on VFA holding Rb. capsulatus, and (vi) Rb. capsulatus (isolate v). The VFA mixture elevated growth rates with a factor 1.32.5 compared to individual VFA. Rb. capsulatus showed the highest growth rates: 1.82.2 d−1 (enriched) and 2.33.8 d−1 (isolated). In a photobioreactor (PBR) inoculated with the Rb. capsulatus enrichment, decreasing sludge retention time (SRT) yielded lower biomass concentrations, yet increased productivities, reaching 1.7 g dry weight (DW) L−1 d−1, the highest phototrophic rate reported thus far, and a growth rate of up to 5 d−1. PNSB represented 2657% of the community and the diversity index was low (37), with a dominance of Rhodopseudomonas at long SRT and Rhodobacter at short SRT. The biomass yield for all cultures, in batch and reactor cultivation, approached 1 g CODBiomass g−1 CODRemoved. An economic estimation for a two-stage approach on brewery wastewater (load 2427 kg COD d−1) showed that 0.5 d SRT allowed for the lowest production cost ( 10 kg−1 DW; equal shares for capex and opex). The findings strengthen the potential for a novel two-stage approach for resource recovery from industrial wastewater, enabling high-rate PNSB production. |
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Wos |
000458223900013 |
Publication Date |
2018-12-27 |
| Series Editor |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
0043-1354; 1879-2448 |
ISBN |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
| Impact Factor |
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Times cited |
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Open Access |
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| Notes |
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Approved |
no |
| Call Number |
UA @ admin @ c:irua:156462 |
Serial |
8739 |
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| Author |
Lindeboom, R.E.F.; Clauwaert, P.; Alloul, A.; Coessens, W.; Christiaens, M.; Vanoppen, M.; Rabaey, K.; Verliefde, A.R.D.; Vlaeminck, S.E. |
| Title |
Water and nutrient recovery from combined urine and grey water treatment in Space |
Type |
P3 Proceeding |
| Year |
2015 |
Publication |
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Abbreviated Journal |
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| Volume |
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Issue |
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Pages |
3 p.
T2 - IWA Resource Recovery Conference, 30 Aug |
| Keywords |
P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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Series Issue |
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Edition |
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ISBN |
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Additional Links |
UA library record |
| Impact Factor |
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Times cited |
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Open Access |
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| Notes |
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Approved |
no |
| Call Number |
UA @ admin @ c:irua:151143 |
Serial |
8747 |
| Permanent link to this record |
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| |
| Author |
Han, M.; Seuntjens, D.; Al-Omari, A.; Takacs, I.; Meerburg, F.; Murthy, S.; Vlaeminck, S.E.; De Clippeleir, H. |
| Title |
Water and process parameters as controllers for the ammonia to nitrite oxidation rate ratio in activated sludge |
Type |
P3 Proceeding |
| Year |
2017 |
Publication |
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Abbreviated Journal |
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| Volume |
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Issue |
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Pages |
3 p.
T2 - IWA 2017 Conference on Sustainable Waste |
| Keywords |
P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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Edition |
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ISBN |
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Additional Links |
UA library record |
| Impact Factor |
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Times cited |
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Open Access |
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| Notes |
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Approved |
no |
| Call Number |
UA @ admin @ c:irua:151110 |
Serial |
8748 |
| Permanent link to this record |
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| Author |
de Paepe, J.; Lindeboom, R.E.F.; Vanoppen, M.; Alonso Farinas, B.; Coessens, W.; Abbas, A.; Christiaens, M.; Dotremont, C.; Beckers, H.; Lamaze, B.; Demey, D.; Rabaey, K.; Clauwaert, P.; Verliefde, A.R.D.; Vlaeminck, S.E. |
| Title |
Water treatment unit breadboard : ground test facility for the recycling of urine and shower water for one astronaut |
Type |
P3 Proceeding |
| Year |
2017 |
Publication |
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Abbreviated Journal |
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| Volume |
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Issue |
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Pages |
2 p.
T2 - 5th IWA Benelux Young Water Professional |
| Keywords |
P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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ISBN |
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Additional Links |
UA library record |
| Impact Factor |
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Times cited |
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Open Access |
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| Notes |
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Approved |
no |
| Call Number |
UA @ admin @ c:irua:151114 |
Serial |
8749 |
| Permanent link to this record |
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| Author |
Faust, V.; Boon, N.; Ganigué, R.; Vlaeminck, S.E.; Udert, K.M. |
| Title |
Optimizing control strategies for urine nitrification : narrow pH control band enhances process stability and reduces nitrous oxide emissions |
Type |
A1 Journal article |
| Year |
2023 |
Publication |
Frontiers in environmental science |
Abbreviated Journal |
|
| Volume |
11 |
Issue |
|
Pages |
1275152-14 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
Nitrification is well-suited for urine stabilization. No base dosage is required if the pH is controlled within an appropriate operating range by urine feeding, producing an ammonium-nitrate fertilizer. However, the process is highly dependent on the selected pH set-points and is susceptible to process failures such as nitrite accumulation or the growth of acid-tolerant ammonia-oxidizing bacteria. To address the need for a robust and reliable process in decentralized applications, two different strategies were tested: operating a two-position pH controller (inflow on/off) with a narrow pH control band at 6.20/6.25 (∆pH = 0.05, narrow-pH) vs. a wider pH control band at 6.00/6.50 (∆pH = 0.50, wide-pH). These variations in pH also cause variations in the chemical speciation of ammonia and nitrite and, as shown, the microbial production of nitrite. It was hypothesized that the higher fluctuations would result in greater microbial diversity and, thus, a more robust process. The diversity of nitrifiers was higher in the wide-pH reactor, while the diversity of the entire microbiome was similar in both systems. However, the wide-pH reactor was more susceptible to tested process disturbances caused by increasing pH or temperature, decreasing dissolved oxygen, or an influent stop. In addition, with an emission factor of 0.47%, the nitrous oxide (N2O) emissions from the wide-pH reactor were twice as high as the N2O emissions from the narrow-pH reactor, most likely due to the nitrite fluctuations. Based on these results, a narrow control band is recommended for pH control in urine nitrification. |
| Address |
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| Corporate Author |
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Thesis |
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| Publisher |
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Place of Publication |
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Editor |
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| Language |
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Wos |
001087861500001 |
Publication Date |
2023-10-10 |
| Series Editor |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
2296-665x |
ISBN |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
| Impact Factor |
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Times cited |
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Open Access |
OpenAccess |
| Notes |
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Approved |
Most recent IF: NA |
| Call Number |
UA @ admin @ c:irua:199585 |
Serial |
8909 |
| Permanent link to this record |
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| Author |
De Micco, V.; Amitrano, C.; Mastroleo, F.; Aronne, G.; Battistelli, A.; Carnero-Diaz, E.; De Pascale, S.; Detrell, G.; Dussap, C.-G.; Ganigué, R.; Jakobsen, Ø.M.; Poulet, L.; Van Houdt, R.; Verseux, C.; Vlaeminck, S.E.; Willaert, R.; Leys, N. |
| Title |
Plant and microbial science and technology as cornerstones to Bioregenerative Life Support Systems in space |
Type |
A1 Journal article |
| Year |
2023 |
Publication |
NPJ microgravity |
Abbreviated Journal |
|
| Volume |
9 |
Issue |
1 |
Pages |
69-12 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
Long-term human space exploration missions require environmental control and closed Life Support Systems (LSS) capable of producing and recycling resources, thus fulfilling all the essential metabolic needs for human survival in harsh space environments, both during travel and on orbital/planetary stations. This will become increasingly necessary as missions reach farther away from Earth, thereby limiting the technical and economic feasibility of resupplying resources from Earth. Further incorporation of biological elements into state-of-the-art (mostly abiotic) LSS, leading to bioregenerative LSS (BLSS), is needed for additional resource recovery, food production, and waste treatment solutions, and to enable more self-sustainable missions to the Moon and Mars. There is a whole suite of functions crucial to sustain human presence in Low Earth Orbit (LEO) and successful settlement on Moon or Mars such as environmental control, air regeneration, waste management, water supply, food production, cabin/habitat pressurization, radiation protection, energy supply, and means for transportation, communication, and recreation. In this paper, we focus on air, water and food production, and waste management, and address some aspects of radiation protection and recreation. We briefly discuss existing knowledge, highlight open gaps, and propose possible future experiments in the short-, medium-, and long-term to achieve the targets of crewed space exploration also leading to possible benefits on Earth. |
| Address |
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| Corporate Author |
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Thesis |
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Place of Publication |
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Editor |
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Wos |
001093834300001 |
Publication Date |
2023-08-24 |
| Series Editor |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
2373-8065 |
ISBN |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
| Impact Factor |
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Times cited |
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Open Access |
OpenAccess |
| Notes |
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Approved |
Most recent IF: NA |
| Call Number |
UA @ admin @ c:irua:199050 |
Serial |
8916 |
| Permanent link to this record |
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| Author |
Faust, V.; Vlaeminck, S.E.; Ganigué, R.; Udert, K.M. |
| Title |
Influence of pH on urine nitrification : community shifts of ammonia-oxidizing bacteria and inhibition of nitrite-oxidizing bacteria |
Type |
A1 Journal article |
| Year |
2024 |
Publication |
ACS ES&T engineering |
Abbreviated Journal |
|
| Volume |
4 |
Issue |
2 |
Pages |
342-353 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
Urine nitrification is pH-sensitive due to limited alkalinity and high residual ammonium concentrations. This study aimed to investigate how the pH affects nitrogen conversion and the microbial community of urine nitrification with a pH-based feeding strategy. First, kinetic parameters for NH3, HNO2, and NO2– limitation and inhibition were determined for nitrifiers from a urine nitrification reactor. The turning point for ammonia-oxidizing bacteria (AOB), i.e., the substrate concentration at which a further increase would lead to a decrease in activity due to inhibitory effects, was at an NH3 concentration of 12 mg-N L–1, which was reached only at pH values above 7. The total nitrite turning point for nitrite-oxidizing bacteria (NOB) was pH-dependent, e.g., 18 mg-N L–1 at pH 6.3. Second, four years of data from two 120 L reactors were analyzed, showing that stable nitrification with low nitrite was most likely between pH 5.8 and 6.7. And third, six 12 L urine nitrification reactors were operated at total nitrogen concentrations of 1300 and 3600 mg-N L–1 and pH values between 2.5 and 8.5. At pH 6, the AOB Nitrosomonas europaea was found, and the NOB belonged to the genus Nitrobacter. At pH 7, nitrite accumulated, and Nitrosomonas halophila was the dominant AOB. NOB were inhibited by HNO2 accumulation. At pH 8.5, the AOB Nitrosomonas stercoris became dominant, and NH3 inhibited NOB. Without influent, the pH dropped to 2.5 due to the growth of the acid-tolerant AOB “Candidatus Nitrosacidococcus urinae”. In conclusion, pH is a decisive process control parameter for urine nitrification by influencing the selection and kinetics of nitrifiers. |
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Place of Publication |
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Editor |
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Wos |
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Publication Date |
2023-11-02 |
| Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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ISBN |
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Additional Links |
UA library record |
| Impact Factor |
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Times cited |
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Open Access |
Not_Open_Access |
| Notes |
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Approved |
Most recent IF: NA |
| Call Number |
UA @ admin @ c:irua:203306 |
Serial |
9048 |
| Permanent link to this record |
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| Author |
Verbeelen, T.; Fernandez, C.A.; Nguyen, T.H.; Gupta, S.; Aarts, R.; Tabury, K.; Leroy, B.; Wattiez, R.; Vlaeminck, S.E.; Leys, N.; Ganigué, R.; Mastroleo, F. |
| Title |
Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity |
Type |
A1 Journal article |
| Year |
2024 |
Publication |
NPJ microgravity |
Abbreviated Journal |
|
| Volume |
10 |
Issue |
1 |
Pages |
3-19 |
| Keywords |
A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL) |
| Abstract |
Regenerative life support systems (RLSS) will play a vital role in achieving self-sufficiency during long-distance space travel. Urine conversion into a liquid nitrate-based fertilizer is a key process in most RLSS. This study describes the effects of simulated microgravity (SMG) on Comamonas testosteroni, Nitrosomonas europaea, Nitrobacter winogradskyi and a tripartite culture of the three, in the context of nitrogen recovery for the Micro-Ecological Life Support System Alternative (MELiSSA). Rotary cell culture systems (RCCS) and random positioning machines (RPM) were used as SMG analogues. The transcriptional responses of the cultures were elucidated. For CO2-producing C. testosteroni and the tripartite culture, a PermaLifeTM PL-70 cell culture bag mounted on an in-house 3D-printed holder was applied to eliminate air bubble formation during SMG cultivation. Gene expression changes indicated that the fluid dynamics in SMG caused nutrient and O2 limitation. Genes involved in urea hydrolysis and nitrification were minimally affected, while denitrification-related gene expression was increased. The findings highlight potential challenges for nitrogen recovery in space. |
| Address |
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| Corporate Author |
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Thesis |
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| Publisher |
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Place of Publication |
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Editor |
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Wos |
001140007100001 |
Publication Date |
2024-01-10 |
| Series Editor |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
2373-8065 |
ISBN |
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Additional Links |
UA library record; WoS full record |
| Impact Factor |
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Times cited |
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Open Access |
Not_Open_Access |
| Notes |
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Approved |
Most recent IF: NA |
| Call Number |
UA @ admin @ c:irua:202285 |
Serial |
9113 |
| Permanent link to this record |
