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Author | Rather, J.A.; Pilehvar, S.; De Wael, K. | ||||
Title | A graphene oxide amplification platform tagged with tyrosinase-zinc oxide quantum dot hybrids for the electrochemical sensing of hydroxylated polychlorobiphenyls | Type | A1 Journal article | ||
Year | 2014 | Publication | Sensors and actuators : B : chemical | Abbreviated Journal | Sensor Actuat B-Chem |
Volume | 190 | Issue | Pages | 612-620 | |
Keywords | A1 Journal article; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) | ||||
Abstract | Graphene oxide can act as an amplification platform for the immobilization of a hybrid structure composed of tyrosinase (Tyr) and zinc oxide quantum dots (ZnO QDs). This article describes how this platform increases the sensitivity for the detection of hydroxylated polychlorobiphenyls (OH-PCBs). The adsorption of Tyr (with low isoelectric point) on the positively charged surface of ZnO QDs is based on electrostatic interactions. The scanning electron microscopic images and UVvis spectroscopic analysis demonstrated the adsorption of Tyr on ZnO QDs. The stepwise assembly process of the fabricated biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The synthesized ZnO QDs and graphene oxide were characterized by Raman spectroscopy, infrared spectroscopy, X-ray diffraction and scanning electron microscopic techniques. The determination of OH-PCBs was carried out by using square wave voltammetry over the concentration range of 2.827.65 μM with a detection limit of 0.15 μM with good reproducibility, selectivity and acceptable stability. The high value of surface coverage of ZnO QDs and small value of MichaelisMenten constant (View the MathML source) confirmed an excellent loading of the Tyr and a high affinity of the biosensor toward the detection of OH-PCBs. This biosensor and the described sensing platform offer a great potential for rapid, cost-effective and on-field analysis of OH-PCBs. | ||||
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Publisher | Place of Publication | Editor | |||
Language | Wos | 000326687700082 | Publication Date | 2013-09-14 | |
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ISSN | 0925-4005 | ISBN | Additional Links | UA library record; WoS full record; WoS citing articles | |
Impact Factor | 5.401 | Times cited | 26 | Open Access | |
Notes | ; The authors are highly thankful for the mobility grant (Non-Europe Postdoc Fellowship) for one of the author (Jahangir Ahmad Rather) supported by the Belgian Federal Science Policy (Belspo) co-funded by the Marie Curie Actions from the European Commission. Sanaz Pilehvar is funded by BOF-DOCPRO UA. We are also thankful to the EMAT (Electron Microscopy for Materials Science) group and Laboratory of adsorption and catalysis group of the University of Antwerp for the XRD, Raman and FTIR characterization of samples (GO and ZnO QDs). ; | Approved | Most recent IF: 5.401; 2014 IF: 4.097 | ||
Call Number | UA @ admin @ c:irua:110566 | Serial | 5636 | ||
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