|   | 
Details
   web
Records
Author Agrawal, S.; Weissbrodt, D.G.; Annavajhala, M.; Jensen, M.M.; Arroyo, J.M.C.; Wells, G.; Chandran, K.; Vlaeminck, S.E.; Terada, A.; Smets, B.F.; Lackner, S.
Title Time to act–assessing variations in qPCR analyses in biological nitrogen removal with examples from partial nitritation/anammox systems Type A1 Journal article
Year (down) 2021 Publication Water Research Abbreviated Journal Water Res
Volume 190 Issue Pages 116604
Keywords A1 Journal article; Sustainable Energy, Air and Water Technology (DuEL)
Abstract Quantitative PCR (qPCR) is broadly used as the gold standard to quantify microbial community fractions in environmental microbiology and biotechnology. Benchmarking efforts to ensure the comparability of qPCR data for environmental bioprocesses are still scarce. Also, for partial nitritation/anammox (PN/A) systems systematic investigations are still missing, rendering meta-analysis of reported trends and generic insights potentially precarious. We report a baseline investigation of the variability of qPCR-based analyses for microbial communities applied to PN/A systems. Round-robin testing was performed for three PN/A biomass samples in six laboratories, using the respective in-house DNA extraction and qPCR protocols. The concentration of extracted DNA was significantly different between labs, ranged between 2.7 and 328 ng mg−1 wet biomass. The variability among the qPCR abundance data of different labs was very high (1−7 log fold) but differed for different target microbial guilds. DNA extraction caused maximum variation (3–7 log fold), followed by the primers (1–3 log fold). These insights will guide environmental scientists and engineers as well as treatment plant operators in the interpretation of qPCR data.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos 000632807700001 Publication Date 2020-11-05
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0043-1354; 1879-2448 ISBN Additional Links UA library record; WoS full record; WoS citing articles
Impact Factor 6.942 Times cited Open Access OpenAccess
Notes Approved Most recent IF: 6.942
Call Number UA @ admin @ c:irua:173838 Serial 8672
Permanent link to this record
 

 
Author Zhang, Q.; Vlaeminck, S.E.; DeBarbadillo, C.; Su, C.; Al-Omari, A.; Wett, B.; Pümpel, T.; Shaw, A.; Chandran, K.; Murthy, S.; De Clippeleir, H.
Title Supernatant organics from anaerobic digestion after thermal hydrolysis cause direct and/or diffusional activity loss for nitritation and anammox Type A1 Journal article
Year (down) 2018 Publication Water research Abbreviated Journal
Volume 143 Issue Pages 270-281
Keywords A1 Journal article; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract Treatment of sewage sludge with a thermal hydrolysis process (THP) followed by anaerobic digestion (AD) enables to boost biogas production and minimize residual sludge volumes. However, the reject water can cause inhibition to aerobic and anoxic ammonium-oxidizing bacteria (AerAOB & AnAOB), the two key microbial groups involved in the deammonification process. Firstly, a detailed investigation elucidated the impact of different organic fractions present in THP-AD return liquor on AerAOB and AnAOB activity. For AnAOB, soluble compounds linked to THP conditions and AD performance caused the main inhibition. Direct inhibition by dissolved organics was also observed for AerAOB, but could be overcome by treating the filtrate with extended aerobic or anaerobic incubation or with activated carbon. AerAOB additionally suffered from particulate and colloidal organics limiting the diffusion of substrates. This was resolved by improving the dewatering process through an optimized flocculant polymer dose and/or addition of coagulant polymer to better capture the large colloidal fraction, especially in case of unstable AD performance. Secondly, a new inhibition model for AerAOB included diffusion-limiting compounds based on the porter-equation, and achieved the best fit with the experimental data, highlighting that AerAOB were highly sensitive to large colloids. Overall, this paper for the first time provides separate identification of organic fractions within THP-AD filtrate causing differential types of inhibition. Moreover, it highlights the combined effect of the performance of THP, AD and dewatering on the downstream autotrophic nitrogen removal kinetics. (C) 2018 Elsevier Ltd. All rights reserved.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos 000443664000027 Publication Date 2018-06-18
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0043-1354; 1879-2448 ISBN Additional Links UA library record; WoS full record; WoS citing articles
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:152911 Serial 8623
Permanent link to this record
 

 
Author Zhang, Q.; Vlaeminck, S.E.; DeBarbadillo, C.; Suzuki, R.; Kharkar, S.M.; Al-Omari, A.; Wett, B.; Chandran, K.; Murthy, S.; De Clippeleir, H.
Title Startup strategies of deammonification reactors treating reject water from thermally hydrolyzed solids Type P3 Proceeding
Year (down) 2017 Publication Abbreviated Journal
Volume Issue Pages 5 p. T2 - WEFTEC.17, 30 September 4 October 2017,
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151113 Serial 8579
Permanent link to this record
 

 
Author Zhang, Q.; Higgins, M.J.; Vlaeminck, S.E.; DeBarbadillo, C.; Suzuki, R.; Kharkar, S.M.; Al-Omari, A.; Wett, B.; Chandran, K.; Murthy, S.; De Clippeleir, H.
Title Minimizing recalcitrant organics and maximizing nitrogen removal linked to advanced biosolids processing at Blue Plains WWTP Type P3 Proceeding
Year (down) 2017 Publication Abbreviated Journal
Volume Issue Pages 12 p. T2 - IWA 2017 Conference on Sustainable Wast
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151107 Serial 8252
Permanent link to this record
 

 
Author Zhang, Q.; De Clippeleir, H.; Shaw, A.; DeBarbadillo, C.; Su, C.; Al-Omari, A.; Wett, B.; Chandran, K.; Vlaeminck, S.E.; Murthy, S.
Title Mechanistic understanding of microbial activity inhibition : case study on sidestream deammonification for digester supernatant pretreated by thermal hydrolysis Type P3 Proceeding
Year (down) 2016 Publication Abbreviated Journal
Volume Issue Pages 5 p. T2 - WEF/IWA Nutrient Removal and Recovery Co
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151133 Serial 8214
Permanent link to this record
 

 
Author Zhang, Q.; De Clippeleir, H.; DeBarbadillo, C.; Su, C.; Al-Omari, A.; Wett, B.; Chandran, K.; Vlaeminck, S.E.; Murthy, S.
Title Inhibition mechanisms affecting deammonification of dewatering filtrate from thermally hydrolyzed digested solid Type P3 Proceeding
Year (down) 2016 Publication Abbreviated Journal
Volume Issue Pages 9 p. T2 - WEFTEC.16, 24 - 28 September 2016, New O
Keywords P3 Proceeding; Engineering sciences. Technology; Sustainable Energy, Air and Water Technology (DuEL)
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos Publication Date
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record
Impact Factor Times cited Open Access
Notes Approved no
Call Number UA @ admin @ c:irua:151123 Serial 8102
Permanent link to this record