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“Electroactivity of superoxide anion in aqueous phosphate buffers analyzed with platinized microelectrodes”. Lefrancois P, Girard-Sahun F, Badets V, Clement F, Arbault S, Electroanalysis (2020). http://doi.org/10.1002/ELAN.202060456
Abstract: The reactivity of platinized ultramicroelectrodes (Pt-black UMEs) towards superoxide anion O-2(.-), an unstable Reactive Oxygen Species (ROS), and its relatives, H2O2 and O-2, was studied. Voltammetric studies in PBS demonstrate that Pt-black UMEs provide: i) a well-resolved reversible redox signature for O-2(.-) detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H2O2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt-black surfaces solely yields H2O2 (2 electrons/2 H+) at physiological pH. Consequently, Pt-black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico-chemical investigations.
Keywords: A1 Journal article; Plasma Lab for Applications in Sustainability and Medicine – Antwerp (PLASMANT)
Impact Factor: 3
DOI: 10.1002/ELAN.202060456
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“Electropolymerized o-phenylenediamine on graphite promoting the electrochemical detection of nafcillin”. Bottari F, Moro G, Sleegers N, Florea A, Cowen T, Piletsky S, van Nuijs ALN, De Wael K, Electroanalysis 32, 135 (2019). http://doi.org/10.1002/ELAN.201900397
Abstract: By combining molecular modelling and electrochemistry we envision the creation of modified electrodes tailored for a more sensitive and selective detection of a single analyte. In this study we report on a graphite screen printed electrode modified with electropolymerized o-phenylenediamine, selected by rational design, which promotes the detection of nafcillin (NAF), an antibiotic. Parameters such as monomer concentration, pH and number of electropolymerization cycles were optimized to obtain the highest current signal for the target upon amperometric detection. NAF identification was based on the redox process at +1.1 V (vs pseudo Ag), ascribed to the oxidation of the C-7 side chain. With the optimized modification protocol, a two-fold increase in nafcillin signal could be obtained: the calibration plot in 0.1 M Britton-Robinson buffer pH 4 showed a limit of detection of 80 nM with improved sensitivity and reproducibility (RSD<5 %) compared to the detection at non-modified electrodes.
Keywords: A1 Journal article; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Toxicological Centre
Impact Factor: 2.851
Times cited: 1
DOI: 10.1002/ELAN.201900397
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“Intercalation of proflavine in ssDNA aptamers : effect on binding of the specific target chloramphenicol”. Pilehvar S, Jambrec D, Gebala M, Schuhmann W, De Wael K, Electroanalysis 27, 1836 (2015). http://doi.org/10.1002/ELAN.201500192
Abstract: The structural modification of ssDNA-based aptamers upon specific binding of its target molecule leads to changes of the charge-transfer resistance (Rct) of a negatively-charged free-diffusing redox probe. The aptamer adopts a structure due to self-hybridization which is stabilized using profalvine as intercalator. The pre-organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the Rct values. Pre-incubation of the aptamer-modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP-dependent variation of the Rct values.
Keywords: A1 Journal article; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Impact Factor: 2.851
Times cited: 5
DOI: 10.1002/ELAN.201500192
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“Label-free impedance aptasensor for major peanut allergen Ara h 1”. Trashin S, De Jong M, Breugelmans T, Pilehvar S, De Wael K, Electroanalysis 27, 32 (2015). http://doi.org/10.1002/ELAN.201400365
Abstract: The peanut allergen Ara h 1 belonging to the vicilin family of seed storage proteins is known to be a suitable marker for peanut detection in foods. In the present work, we suggest an electrochemical detection of Ara h 1 using a recently selected 80-base DNA aptamer. The detection strategy relies on insulation effect of a bulky protein captured by an immobilized aptamer. The electrodes were modified by the aptamer and characterized using electrochemical impedance spectroscopy (EIS). The aptamer surface density and analytical parameters of the calibration curves for Ara h 1 were compared with the system prepared with thrombin binding aptamer (TBA) and operated in the same conditions.
Keywords: A1 Journal article; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Applied Electrochemistry & Catalysis (ELCAT)
Times cited: 10
DOI: 10.1002/ELAN.201400365
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“Fullerene-\beta-cyclodextrin conjugate based electrochemical sensing device for ultrasensitive detection of p-nitrophenol”. Rather JA, Debnath P, De Wael K, Electroanalysis 25, 2145 (2013). http://doi.org/10.1002/ELAN.201300304
Abstract: The article describes the use of a fullerene (C60)-β-cyclodextrin conjugate, synthesized via 1,3-dipolar cycloaddition, for the ultrasensitive electrochemical detection of p-nitrophenol. This conjugate was successfully immobilized on the surface of a glassy carbon electrode and the developed device showed high activity towards p-nitrophenol due to the synergetic effect of C60, the latter becoming highly conductive upon reduction. The determination of p-nitrophenol was performed by using square wave voltammetry over a concentration range from 2.8×10−9 mol L−1 to 4.2×10−7 mol L−1 and the detection limit was calculated to be 1.2×10−9 mol L−1.
Keywords: A1 Journal article; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Impact Factor: 2.851
Times cited: 13
DOI: 10.1002/ELAN.201300304
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“Highly sensitive and selective voltammetric sensor fullerene modified glassy carbon electrode for determination of cefitizoxime in solubilized system”. Jain R, Rather JA, Dwivedi A, Vikas, Electroanalysis 22, 2600 (2010). http://doi.org/10.1002/ELAN.201000243
Abstract: The usefulness of fullerene modified glassy carbon electrode in mediating the reduction of cefitizoxime in solubilized system has been demonstrated. Due to the unique structure and extraordinary properties, fullerene shows higher catalytic efficiency towards cefitizoxime reduction. The kinetic parameters, electron transfer coefficient (α) and rate constant (K0) across the modified electrode are 0.37 and 0.1081/s respectively. The proposed square-wave voltammetric method is linear over the concentration range 1.210.3 µg/mL. The limit of detection (LOD) is found 0.27 ng/mL. High sensitivity and selectivity together with low detection limit of the electrode response make it suitable for the determination of cefitizoxime.
Keywords: A1 Journal article; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
DOI: 10.1002/ELAN.201000243
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