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Author Trashin, S.; De Jong, M.; Breugelmans, T.; Pilehvar, S.; De Wael, K.
Title Label-free impedance aptasensor for major peanut allergen Ara h 1 Type A1 Journal article
Year (down) 2015 Publication Electroanalysis Abbreviated Journal
Volume 27 Issue 1 Pages 32-37
Keywords A1 Journal article; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Applied Electrochemistry & Catalysis (ELCAT)
Abstract The peanut allergen Ara h 1 belonging to the vicilin family of seed storage proteins is known to be a suitable marker for peanut detection in foods. In the present work, we suggest an electrochemical detection of Ara h 1 using a recently selected 80-base DNA aptamer. The detection strategy relies on insulation effect of a bulky protein captured by an immobilized aptamer. The electrodes were modified by the aptamer and characterized using electrochemical impedance spectroscopy (EIS). The aptamer surface density and analytical parameters of the calibration curves for Ara h 1 were compared with the system prepared with thrombin binding aptamer (TBA) and operated in the same conditions.
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Corporate Author Thesis
Publisher Place of Publication Editor
Language Wos 000347838300004 Publication Date 2014-10-13
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Additional Links UA library record; WoS full record; WoS citing articles
Impact Factor Times cited 10 Open Access
Notes ; ST greatly appreciates the Research Council University of Antwerp (IWS BOF UA) for funding the post-doctoral fellowship. ; Approved Most recent IF: NA
Call Number UA @ admin @ c:irua:119442 Serial 5686
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