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Author |
Van Echelpoel, R.; De Wael, K. |
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Title |
Voltammetric drug testing makes sense at the border |
Type |
A1 Journal article |
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Year |
2024 |
Publication |
Nature Reviews Chemistry |
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Pages  |
1-2 |
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Keywords |
A1 Journal article; Engineering sciences. Technology; Antwerp Electrochemical and Analytical Sciences Lab (A-Sense Lab) |
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Abstract |
The European BorderSens project leverages voltammetric sensors, developed with end-users' input, to rapidly and accurately detect illicit drugs. By embracing practicalities and validation, this technology has the potential to combat the illicit drug problem. |
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001142000900001 |
Publication Date |
2024-01-12 |
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2397-3358 |
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UA library record; WoS full record |
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Not_Open_Access |
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no |
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Call Number |
UA @ admin @ c:irua:202646 |
Serial |
9112 |
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Author |
Blust, R.J.P.; Dardenne, F.; De Wael, K.; Nagels, L.; Van Camp, G. |
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Title |
Electrochemical aptasensors with a gelatin B matrix : EP13175128 |
Type |
Patent |
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Year |
2013 |
Publication |
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Patent; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
An aptamer-based electrochemical sensor, wherein said aptamer is covalently bonded to or chemisorbed on an electrode, said aptamer forming a robust complex with a target molecule and is encapsulated by a gelatin B matrix; a method of manufacturing an aptamer-based electrochemical sensor for determining a concentration of a target molecule comprising the steps of: selecting an aptamer to form a robust complex with a target molecule using the SELEX procedure; synthesizing said aptamer; adsorbing said aptamer on or covalently coupling said aptamer with an electrode; and providing a gelatin B matrix for said aptamer on said electrode thereby realising said aptamer-based potentiometric or amperometric sensor; and the use of the aptamer-based electrochemical sensor produced according to the above method for the electrochemical determination of a concentration of 15 the target molecule. |
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Most recent IF: NA |
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Call Number |
UA @ admin @ c:irua:109364 |
Serial |
5587 |
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Author |
De Wael, K.; Nagels, L.; Van Camp, G. |
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Title |
Potentiometric sensors and method for measuring intermolecular interactions |
Type |
Patent |
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Year |
2013 |
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Keywords |
Patent; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Most recent IF: NA |
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Call Number |
UA @ admin @ c:irua:111264 |
Serial |
5779 |
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Author |
de Jong, M.; Florea, A.; Daems, D.; Van Loon, J.; Samyn, N.; De Wael, K. |
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Title |
Electrochemical Analysis of Speedball-like Polydrug Samples |
Type |
A1 Journal article |
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Year |
2020 |
Publication |
Analyst |
Abbreviated Journal |
Analyst |
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Keywords |
A1 Journal article; Pharmacology. Therapy; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Product development |
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Abstract |
Increasing global production, trafficking and consumption of drugs of abuse cause an emerging threat to people’s health and safety. Electrochemical approaches have proven to be useful for on-site analysis of drugs of abuse. However, few attention has been focused on the analysis of polydrug samples, despite these samples causing severe health concerns, certainly when stimulants and depressants are combined, as is the case for Speedball, a mixture of cocaine and heroin. In this work, we provide solutions for the selective detection of cocaine (stimulant) in polydrug samples adulterated with heroin and codeine (depressants). The presence of either one of these compounds in cocaine street samples leads to an overlap with the cocaine signal in square-wave voltammetry measurements at unmodified carbon screen-printed electrodes, leading to inconclusive screening results in the field. The provided solutions to this problem consist of two parallel approaches: (i) cathodic pretreatment of the carbon screen-printed electrode surface prior to measurement in both alkaline and neutral conditions; (ii) electropolymerization of orthophenylenediamine on graphene modified carbon screen-printed electrodes prior to measurement in neutral conditions. Both strategies allow simultaneous detection of cocaine and heroin in speedball samples as well as simultaneous detection of cocaine and codeine. Implementing these strategies in portable devices holds great potential for significantly improved accuracy of on-site cocaine screening in polydrug samples. |
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000568961600011 |
Publication Date |
2020-07-28 |
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ISSN |
0003-2654 |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
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Impact Factor |
4.2 |
Times cited |
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Open Access |
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Notes |
This work was supported by IOF-SBO and IOF-POC from University of Antwerp, Antwerp, Belgium; and VLAIO IM [HBC.2019.2181], Brussels, Belgium. |
Approved |
Most recent IF: 4.2; 2020 IF: 3.885 |
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Call Number |
AXES @ axes @c:irua:170444 |
Serial |
6395 |
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Author |
Bottari, F.; Daems, E.; de Vries, A.-M.; Van Wielendaele, P.; Trashin, S.; Blust, R.; Sobott, F.; Madder, A.; Martins, J.C.; De Wael, K. |
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Title |
Do aptamers always bind? The need for a multifaceted analytical approach when demonstrating binding affinity between aptamer and low molecular weight compounds |
Type |
A1 Journal article |
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Year |
2020 |
Publication |
Journal Of The American Chemical Society |
Abbreviated Journal |
J Am Chem Soc |
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Volume |
142 |
Issue |
46 |
Pages |
jacs.0c08691-19630 |
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Keywords |
A1 Journal article; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Medical Biochemistry |
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In this manuscript, we compare different analytical methodologies to validate or disprove the binding capabilities of aptamer sequences. This was prompted by the lack of a universally accepted and robust quality control protocol for the characterization of aptamer performances coupled with the observation of independent yet inconsistent data sets in the literature. As an example, we chose three aptamers with a reported affinity in the nanomolar range for ampicillin, a β-lactam antibiotic, used as biorecognition elements in several detection strategies described in the literature. Application of a well-known colorimetric assay based on aggregation of gold nanoparticles (AuNPs) yielded conflicting results with respect to the original report. Therefore, ampicillin binding was evaluated in solution using isothermal titration calorimetry (ITC), native nano-electrospray ionization mass spectrometry (native nESI-MS), and 1H-nuclear magnetic resonance spectroscopy (1H NMR). By coupling the thermodynamic data obtained with ITC with the structural information on the binding event given by native nESI-MS and 1H NMR we could verify that none of the ampicillin aptamers show any specific binding with their intended target. The effect of AuNPs on the binding event was studied by both ITC and 1H NMR, again without providing positive evidence of ampicillin binding. To validate the performance of our analytical approach, we investigated two well-characterized aptamers for cocaine/quinine (MN4), chosen for its nanomolar range affinity, and l-argininamide (1OLD) to show the versatility of our approach. The results clearly indicate the need for a multifaceted analytical approach, to unequivocally establish the actual detection potential and performance of aptamers aimed at small organic molecules. |
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Wos |
000592911000024 |
Publication Date |
2020-11-09 |
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ISSN |
0002-7863 |
ISBN |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
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Impact Factor |
15 |
Times cited |
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Open Access |
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Notes |
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Approved |
Most recent IF: 15; 2020 IF: 13.858 |
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Call Number |
UA @ admin @ c:irua:173136 |
Serial |
6488 |
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Author |
Thiruvottriyur Shanmugam, S.; Trashin, S.; De Wael, K. |
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Title |
Gold-sputtered microelectrodes with built-in gold reference and counter electrodes for electrochemical DNA detection |
Type |
A1 Journal article |
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Year |
2020 |
Publication |
Analyst |
Abbreviated Journal |
Analyst |
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Volume |
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Pages |
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Keywords |
A1 Journal article; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation) |
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Abstract |
Gold-sputtered microelectrodes with built-in gold reference and counter electrodes represent a promising platform for the development of disposable DNA sensors. Pretreating gold electrode surfaces and immobilization of DNA thereon is commonly employed in biosensing applications. However, with no scientific or practical guidelines to prepare a DNA sensor using these miniature gold-sputtered microelectrodes, cleaning and immobilization steps need to be systematically optimized and updated. In this work, we present efficient cleaning and modification of miniaturized gold-sputtered microelectrodes with thiolated DNA probes for DNA detection. Additional discussions on subtleties and nuances involved at each stage of pretreating and modifying gold-sputtered microelectrodes are included to present a robust, well-founded protocol. It was evident that the insights on cleaning polycrystalline gold disk electrodes with a benchmark electrode surface for DNA sensors, cannot be transferred to clean these miniature gold-sputtered microelectrodes. Therefore, a comparison between five different cleaning protocols was made to find the optimal one for gold-sputtered microelectrodes. Additionally, two principally different immobilization techniques for gold-sputtered microelectrode modification with thiolated ssDNA were compared i.e., immobilization through passive chemisorption and potential perturbation were compared in terms of thiol-specific attachment and thiol-unspecific adsorption through nitrogenous bases. The hybridization performance of these prepared electrodes was characterized by their sensitive complementary DNA capturing ability, detected by a standard alkaline phosphatase assay. Immobilization through passive chemisorption proved to be efficient in capturing the complementary target DNA with a detection limit of 0.14 nM and sensitivity of 9.38 A M−1 cm2. In general, this work presents a comprehensive understanding of cleaning, modification and performance of gold-sputtered microelectrodes with built-in gold reference and counter electrodes for both fundamental investigations and practical DNA sensing applications. |
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Wos |
000592315100017 |
Publication Date |
2020-09-17 |
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ISSN |
0003-2654 |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
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Impact Factor |
4.2 |
Times cited |
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Open Access |
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Approved |
Most recent IF: 4.2; 2020 IF: 3.885 |
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Call Number |
UA @ admin @ c:irua:172447 |
Serial |
6527 |
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Author |
Van Loon, J.; De Jong, M.; De Wael, K.; Du Bois, E. |
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Title |
Transposing testing from lab to on-site environment : a case of cocaine powder sampling |
Type |
P3 Proceeding |
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Year |
2020 |
Publication |
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Abbreviated Journal |
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P3 Proceeding; Engineering sciences. Technology; AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation); Product development |
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Abstract |
This paper reports on the transition process to convert a specific lab technique to the on-site, real-life environment. Bringing a lab test to an on-site environment involves many difficulties regarding to the context, people skills and environmental support. Within this project, a case about sampling for an electrochemical measurement, was investigated as a basis to reason upon some general conclusions related to the process of transposing lab-tests to an on-site environment. The current lab test was analysed and compared with a focus group discussion with future users. Based on the findings, assumptions for the new sampling technique in the specific case were formulated. New low-tech methods to achieve the sampling were extensively tested and verified. Starting from this chosen case an argumentation was set up to generalise the conclusions, by reasoning on other cases of products that already passed this transition to the field. Based on a comparison, we could discuss that the selected parameters related to impact of the context environment, of the people that should handle the tests, related to the reasons for transposing the technology, and related to the technology that will be transposed to the on-site environment, were of importance of almost all cases and can therefore be considered as context-independent and related to the transfer to on-site testing. |
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978-94-6384-131-3 |
Additional Links |
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Most recent IF: NA |
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Call Number |
UA @ admin @ c:irua:169896 |
Serial |
6637 |
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Author |
Parrilla, M.; Vanhooydonck, A.; Watts, R.; De Wael, K. |
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Title |
Wearable wristband-based electrochemical sensor for the detection of phenylalanine in biofluids |
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A1 Journal article |
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Year |
2022 |
Publication |
Biosensors and bioelectronics |
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197 |
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Keywords |
A1 Journal article; Engineering sciences. Technology; Product development; Antwerp Electrochemical and Analytical Sciences Lab (A-Sense Lab) |
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Abstract |
Wearable electrochemical sensors are driven by the user-friendly capability of on-site detection of key biomarkers for health management. Despite the advances in biomolecule monitoring such as glucose, still, several unmet clinical challenges need to be addressed. For example, patients suffering from phenylketonuria (PKU) should be able to monitor their phenylalanine (PHE) level in a rapid, decentralized, and affordable manner to avoid high levels of PHE in the body which can lead to a profound and irreversible mental disability. Herein, we report a wearable wristband electrochemical sensor for the monitoring of PHE tackling the necessity of controlling PHE levels in PHE hydroxylase deficiency patients. The proposed electrochemical sensor is based on a screen-printed electrode (SPE) modified with a membrane consisting of Nafion, to avoid interferences in biofluids. The membrane also consists of sodium 1,2-naphthoquinone-4-sulphonate for the in situ derivatization of PHE into an electroactive product, allowing its electrochemical oxidation at the surface of the SPE in alkaline conditions. Importantly, the electrochemical sensor is integrated into a wristband configuration to enhance user interaction and engage the patient with PHE self-monitoring. Besides, a paper-based sampling strategy is designed to alkalinize the real sample without the need for sample pretreatment, and thus simplify the analytical process. Finally, the wearable device is tested for the determination of PHE in saliva and blood serum. The proposed wristband-based sensor is expected to impact the PKU self-monitoring, facilitating the daily lives of PKU patients toward optimal therapy and disease management. |
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000719366400003 |
Publication Date |
2021-11-02 |
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ISSN |
0956-5663 |
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Additional Links |
UA library record; WoS full record; WoS citing articles |
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Times cited |
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Open Access |
OpenAccess |
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no |
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Call Number |
UA @ admin @ c:irua:183086 |
Serial |
8957 |
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